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. Author manuscript; available in PMC: 2021 Sep 10.
Published in final edited form as: Cell Rep. 2021 Aug 24;36(8):109602. doi: 10.1016/j.celrep.2021.109602

Figure 6. ZEB1 promotes Th17 differentiation by repressing miR-101-3p that inhibits JAK2 expression.

Figure 6.

(A) Schematic displaying a model by which ZEB1 promotes JAK2 expression through repressing miRNA expression.

(B) Heatmap showing the expression of miRNAs, which putatively target JAK2, in Th17 cells from siCTL and siZEB1 cohorts (miRNAs with a padj < 0.1 were marked in red).

(C) Schematic of the experimental design to assess the necessity and sufficiency of siZEB1-dependent loss of JAK2 in Th17 differentiation condition.

(D) Western blots show that miR-101-3p is necessary and sufficient for siZEB1-dependent loss of JAK2 under Th17 differentiation conditions. Representative result from 3 independent experiments.

(E and F) Flow cytometry evaluating Th17 differentiation of human CD4+ naive T cells transfected with siCTL (E) or siZEB1 (F) and miRNA mimic (m101-3p or scrambled control, mCTL) or a hairpin inhibitor (in101-3p or scrambled control, inCTL). The contour plots are representative 3 different human donors, summarized in the graph at right.

All statistical differences in (E) and (F) were tested using paired Student’s t test (two-tailed). NS, not significant; *p < 0.05, **p < 0.01.