Table 2.
Estimation statistics table showing effect size (unpaired means difference) with 95% CIs and permutation tests p values as follows: effect size [CI width with lower bound, upper bound]
| Figure | Panel | Measure | Groups (sample size) | Effect size [CI width] | Permutation p value |
|---|---|---|---|---|---|
| 1 | E | Effect of secretory IgM on the % area of IgG deposition at 2 weeks in select sections (−1200, −600, 600 μm). | KO (4 mice, 3 sections each) |
31.6 [95%CI 18.6, 43.4] | 0.0108 |
| WT (5 mice, 3 sections each) | |||||
| F | Effect of secretory IgM on the % area of IgG and C3b co-localization at 2 weeks in select sections (−1200, −600, 600 μm). | KO (4 mice, 3 sections each) |
14.9 [95%CI 5.07, 23.4] | 0.03 | |
| WT (5 mice, 3 sections each) | |||||
| G | Effect of secretory IgM on the % area of C3b deposition at 2 weeks in select sections (−1200, −600, 600 μm). | KO (4 mice, 3 sections each) |
12.5 [95%CI 1.48, 23.1] | 0.116 | |
| WT (5 mice, 3 sections each) | |||||
| H | Effects of secretory IgM on levels of serum IgG immunoglobulin | KO: BSL (9), 2 weeks (5), 10 weeks (7) mice |
KO-2-SCI minus KO-BSL: 6.09 [95%CI 3.51, 8.99] |
0.0004 | |
| KO-10-SCI minus KO-BSL: 4.62 [95%CI 1.95, 8.39] |
0.004 | ||||
| WT: BSL (10), 2 weeks (6), 10 weeks (6) mice |
WT-2-SCI minus WT-BSL: 9.36 [95%CI 4.02, 14.6] |
0.0016 | |||
| WT-10-SCI minus WT-BSL: 3.96 [95%CI 0.703, 7.71] |
0.0228 | ||||
| I | Levels of serum IgM immunoglobulin in WT mice at BSL (pre-SCI) and 2 weeks post-SCI | WT: BSL (6), 2 weeks (7) mice |
0.256 [95%CI 0.1, 0.416] | 0.0146 | |
| 3 | E | Effect of secretory IgM on expansion of myelin engulfing macrophages in spinal cord sections with largest IgG deposition. | KO (4 mice, 3 sections each) |
4.24 [95%CI 1.07, 7.19] | 0.0246 |
| WT (5 mice, 3 sections each) | |||||
| 5 | A | Effect of secretory IgM on BMS scores at 2 weeks post-SCI | KO (7), WT (7) mice | −0.286 [95%CI −1.86, 0.643] | 0.575 |
| Effect of secretory IgM on grip strength at 2 weeks post-SCI | KO (7), WT (7) mice | 3.74 [95%CI −6.4, 13.3] | 0.485 | ||
| 5 | C | Effect of secretory IgM on step sequence regularity at 4, 8, and 10 weeks post-SCI | 4 weeks: KO SCI (10), KO sham (10), WT SCI (7), WT sham (10) mice |
KO SCI vs WT SCI −3.95 [95%CI −23.1, 16.8] |
0.739 |
| 8 weeks: KO SCI (10), KO sham (8), WT SCI (7), WT sham (9) mice |
KO SCI vs WT SCI −22.5 [95%CI −38.6, −8.21] |
0.0196 | |||
| 10 weeks: KO SCI (7), KO sham (8), WT SCI (6), WT sham (8) mice |
KO SCI vs WT SCI 31.4 [95.0%CI −49.4, −12.2] |
0.0188 | |||
| D | Effect of secretory IgM on hindlimb swing speed at 4, 8, and 10 weeks post-SCI | 4 weeks: KO SCI (10), KO sham (10), WT SCI (7), WT sham (10) mice |
KO SCI vs WT SCI −7.22 [95%CI −20.9, 4.97] |
0.358 | |
| 8 weeks: KO SCI (12), KO sham (8), WT SCI (7), WT sham (9) mice |
KO SCI vs WT SCI −16.1 [95%CI −34.6, −4.95] |
0.0598 | |||
| 10 weeks: KO SCI (9), KO sham (8), WT SCI (6), WT sham (8) mice |
KO SCI vs WT SCI −18.2 [95%CI −32.9, −3.69] |
0.0412 | |||
| E | Effect of secretory IgM on forelimb swing speed at 4, 8, and 10 weeks post-SCI | 4 weeks: KO SCI (10), KO sham (10), WT SCI (7), WT sham (10) mice |
KO SCI vs WT SCI 0.859 [95%CI −11.3, 12.7] |
0.896 | |
| 8 weeks: KO SCI (12), KO sham (8), WT SCI (7), WT sham (9) mice |
KO SCI vs WT SCI −0.507 [95%CI −8.66, 9.56] |
0.925 | |||
| 10 weeks: KO SCI (9), KO sham (8), WT SCI (6), WT sham (8) mice |
KO SCI vs WT SCI −4.87 [95.0%CI −20.5, 7.37] |
0.524 | |||
| F | Effect of secretory IgM on hindlimb print area at 4, 8, and 10 weeks post-SCI | 4 weeks: KO SCI (10), KO sham (10), WT SCI (7), WT sham (10) mice |
KO SCI vs WT SCI −0.0616 [95%CI −0.175, 0.08] |
0.425 | |
| 8 weeks: KO SCI (12), KO sham (8), WT SCI (7), WT sham (9) mice |
KO SCI vs WT SCI −0.037[95%CI −0.0826, 0.024] |
0.289. | |||
| 5 | F | Effect of secretory IgM on hindlimb print area at 4, 8, and 10 weeks post-SCI | 10 weeks: KO SCI (9), KO sham (8), WT SCI (6), WT sham (8) mice |
KO SCI vs WT SCI −0.096 [95%CI −0.137, −0.0501] |
0.0006 |
| G | Effect of secretory IgM on forelimb print area at 4, 8, and 10 weeks post-SCI | 4 weeks: KO SCI (10), KO sham (10), WT SCI (7), WT sham (10) mice |
KO SCI vs WT SCI −0.0352 [95%CI −0.115, 0.0438] |
0.43 | |
| 8 weeks: KO SCI (12), KO sham (8), WT SCI (7), WT sham (9) mice |
KO SCI vs WT SCI 0.0192 [95%CI −0.0109, 0.055] |
0.352 | |||
| 10 weeks: KO SCI (9), KO sham (8), WT SCI (6), WT sham (8) mice |
KO SCI vs WT SCI −0.023 [95%CI −0.0664, 0.0159] |
0.35 | |||
| H | Effect of secretory IgM on average body speed at 4, 8, and 10 weeks post-SCI | 4 weeks: KO SCI (10), KO sham (10), WT SCI (7), WT sham (10) mice |
KO SCI vs WT SCI 2.39 [95%CI −2.51, 8.17] |
0.424 | |
| 8 weeks: KO SCI (12), KO sham (8), WT SCI (7), WT sham (9) mice |
KO SCI vs WT SCI −3.52 [95%CI −7.53, 1.42] |
0.171 | |||
| 10 weeks: KO SCI (9), KO sham (8), WT SCI (6), WT sham (8) mice |
KO SCI vs WT SCI −2.31 [95%CI −8.24, 3.55] |
0.49 | |||
| 6 | B | Effect of secretory IgM on bacterial load in bladder at 2 weeks post-SCI | KO (7), WT (7) mice | 0.429 [95%CI 0.0, 1.29] | 0 |
| C | Effect of secretory IgM on bacterial load in colon at 2 weeks post-SCI | KO (7), WT (6) mice Upon log-transformation: KO (5), WT (6) mice |
1.29e+02 [95%CI −2.01e+02, 7e+02] | 0.877 | |
| D | Effect of secretory IgM on body weight at 2 weeks post-SCI | KO (7), WT (7) mice | −1.54 [95%CI −7.52, 3.85] | 0.617 | |
| E | Effect of secretory IgM on spleen weight at 2 weeks post-SCI | KO (7), WT (7) mice | 0.005 [95%CI −0.0206, 0.0272] | 0.686 |
p values for permutation tests <0.05 are shown in bold for emphasis.