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. 2021 Sep 10;12:501. doi: 10.1186/s13287-021-02570-9

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© The Author(s) 2021

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 1 — Identification of hAMSCs and hAESCs. a Representative photomicrograph of adherent hAMSCs and hAESCs. Scale bar = 50 μm. b The expressions of markers including Nanog, SOX2 and OCT4 in hAMSCs or hAESCs were analyzed by RT-PCR and water was used as negative control. c The expressions of markers including CD29, CD90, CD73, CD105, CD34, CD45 were analyzed by flow cytometry in hAMSCs or hAESCs. The red lines represent the isotype control, and the blue lines represent the surface markers. d The expressions of markers including HLA-DR, CD80, CD40 and HLA-ABC were analyzed by flow cytometry in hAMSCs or hAESCs. e The expressions of vimentin and CDH1 were detected by immunofluorescence staining in hAMSCs and hAESCs. Scale bar = 20 μm. Data are presented as mean ± SD. The experiments were repeated three times independently and the data of one representative experiment was shown