Fig. 5.

Exosomes derived from hAMSCs inhibited pigmentation by regulating autophagy. B16F10 cells were stimulated with α-MSH and the melanin contents were detected by adding hAMSC-CM that was obtained from hAMSCs treated with or without GW4869 (a). Morphology of exosomes under transmission electron microscopy. Left: magnification =  × 60.0 k, scale bar = 100 nm. Right: magnification =  × 50.0 k, scale bar = 200 nm (b). The expressions of CD63 and CD9 were analyzed by western blotting in hAMSCs-exo (c). Tyrosinase activity was analyzed by treatment with exosomes derived from hAMSCs (d). Melanin content was measured by treatment with exosomes derived from hAMSCs with or without Baf A1 (e). LC3B and P62 were detected by western blotting in B16F10 cells treated with hAMSCs-derived exosomes (f). Data are presented as mean ± SD. The experiments were repeated three times independently and the data of one representative experiment was shown. *p < 0.05; **p < 0.01, ***p < 0.001