Table 1.

HPLC conditions for the analysis of phytochemicals.

Standard	Mobile phase	Detection λ (nm)	Retention Time (min)
Ellagic acid	20 mM phosphate buffer pH 2.5 and acetonitrile (70:30); isocratic elution, flow rate 1.0 mL/min	254	4.945
Gallic acid	20 mM phosphate buffer pH 2.5 and acetonitrile (95:5); isocratic elution, flow rate 1.5 mL/min	270	4.805
Quercetin and Chlorogenic acid	20 mM phosphate buffer pH 2.5 (component A) and acetonitrile (component B); gradient elution, flow rate 1.5 mL/min 0–3 min: 10% B 4–15 min: 10–90% B 16–21 min: 90% B 21–22 min: 90–10% B 22–30 min: 10% B.	255 and 325, respectively	12.677 and 8.626, respectively