REAGENT or RESOURCE	SOURCE	IDENTIFIER
Antibodies
Sp7/Osterix	Abcam	Ab22552; RRID:AB_2194492
Runx2 (D1L7F) Rabbit mAb	Cell Signaling Technology	12556; RRID:AB_2732805
Gli1	Novus Biologicals	Nb600-600; RRID:AB_2111758
Sox9	Millipore Sigma	AB5535; RRID: AB_2239761
Goat anti-rabbit Alexa Fluor 488	Thermo-Fisher Scientific	A27034; RRID:AB_2536097
Goat anti-rabbit Alexa Fluor 647	Thermo-Fisher Scientific	A27040; RRID:AB_2536101
Chemicals, peptides, and recombinant proteins
Tamoxifen	Sigma	T5648
PTH (1-34) (human) Acetate	Bachem Biosciences	H-4835-GMP, 4033364
Tissue-Tek® O.C.T. Compound	Vavantor	4583
GDC-0449	LC laboratories	V-4050
DPBS	Thermo	14190250
FBS	Thermo	26140087
DAPI	Vector Laboratories	H-1200-10
Critical commercial assays
RatLaps ELISA	Immunodiagnostic Systems	AC-02F1
Rat/Mouse PINP EIA Kit	Immunodiagnostic Systems	AC-33F1
Click-iT EdU Alexa Fluor 488 Imaging Kit	Invitrogen	C10337
DeadEndTM fluorometric TUNEL System	Promega	G3250
Deposited data
Raw and analyzed data	This paper	GEO: GSE169560
Experimental models: Organisms/strains
Mouse: Gli1tm3(cre/ERT2)Alj/J (common name: Gli1-CreERT2)	The Jackson Laboratory	007913
Mouse: B6.Cg-Acantm1(cre/ERT2)Crm/J (common name: Acan-CreERT2)	The Jackson Laboratory	019148
Mouse: B6.Cg-Gt(ROSA)26Sortm9(CAG-tdTomato)Hze/J (common name: Ai9)	The Jackson Laboratory	007909
Mouse: B6.Cg-Tg(Col1a1*2.3-GFP)1Rowe/J (common name: ColI-GFP)	The Jackson Laboratory	013134
Mouse: 129-Ihhtm1Amc/J (common name: Ihh+/−)	The Jackson Laboratory	004290
Mouse: B6;129-Igf1rtm2Arge/J (common name: Igf1rc/c)	The Jackson Laboratory	012251
Software and algorithms
BIOQUANT Image Analysis System	BIOQUANT Image Analysis Corporation	BIOQUANT Osteo II
ImageJ	(Schneider et al., 2012)	https://imagej.nih.gov/ij/