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. 2021 Sep 5;10(1):1760–1776. doi: 10.1080/22221751.2021.1971568

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© 2021 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 7: — The impact of NS1 on the replication of H5N8 viruses in mice. Replication of the indicated viruses in mice inoculated intranasally with a low-dose was assessed in 3 mice per group sacrificed at 3 dpi (A-D). Viral RNA was extracted from the indicated organs and was detected using RT-qPCR with standard curves of serial dilutions of B_NS217 (10–100000 pfu) run in each plate. Results are shown as the mean and standard deviation of positive samples (A-D). Asterisks indicate statistical significance based on p values *** < 0.001.