Figure 2.

Variable recoveries of monocyte populations (classical, intermediate, and non-classical). (A) Time series for three representative patients using transplant day: Auto-hematopoietic cell transplantation (HCT) (top panel), Allo-HCT [reduced intensity conditioning (RIC; middle panel)], and Allo-HCT myeloablative conditioning (MAC; bottom panel). The first figure (from left-to-right for each panel) is a cartoon illustration depicting each monocyte subtype, classical (C), intermediate (I), and non-classical (NC) (clockwise from left-to-right) that corresponds to successive multicolor flow cytometry plots that follow. The numbers in each gate are percentages of each monocyte population. The gating strategy is detailed in Figure S1. (B) A boxplot showing the distribution of monocyte subsets determined from a patient’s last blood sample collected. Colored dashed lines indicate the upper limit of “normal” for the percent values for each monocyte subset: 85% classical monocytes (blue), 10% non-classical monocytes (yellow), 5% intermediate monocytes (green). Data points (dots) indicate the monocyte subset type using the same color scheme. (C) A boxplot showing the distribution of absolute monocyte subsets from a patient’s last blood sample and collected and calculated now using the patient’s white blood cell count (K/mcL) taken from the electronic health record; the absolute value (classical, intermediate, non-classical) was determined by taking the white blood cell count (K/mcL) × 1000 × percent cells in gates (B–G; Figure S2).