TABLE 3.
Primers
| Plasmid constructed | Plasmid/gene amplified | Primer | Sequence (5′–3′) |
|---|---|---|---|
| pPK15436 (pRL814-crtIEB) | pRL814 backbone | P1 | ATGTATATCTCCTTCTTAAAGTTAAAC |
| P2 | GCTTGATATCGAATTCCTG | ||
| crtBI of R. sphaeroides | P3 | TTTAAGAAGGAGATATACATATGCCCTCGATCTCGCCC | |
| P4 | TATTTATAATAGAAAGTAAAGACTAGATCGGGTTGGCCCG | ||
| crtE of R. sphaeroides | P5 | CCCGATCTAGTCTTTACTTTCTATTATAAATAAAGGAGACCTTTCATGAGGCACAAGATGGCGTTTGAACAGC | |
| P6 | GCAGGAATTCGATATCAAGCTCAGACGCGGGCCGCGAC | ||
| pPK15404 (pRL814-lacZ) | pRL814 backbone | P7 | CTTAAGGCCGGATCTTGCGGCCCC |
| P8 | TGGGATTACACATGGCATG | ||
| Promoter of sod of Z. mobilis | P9 | GAGGGGCCGCAAGATCCGGCCTTAAGGCTGGGAATAGCATTTCTC | |
| P10 | TCATGGTCATGATGTGATCTCCCAATTC | ||
| lacZ of E. coli MG1655 | P11 | AGATCACATCATGACCATGATTACGGATTCAC | |
| P12 | CCATGCCATGTGTAATCCCAGCGAAATACGGGCAGACATG | ||
| pPK15306 (pRL814-xylA, xylB, rpi and yajO) | pRL814 backbone | P13 | AATTTAGTATATCCTTTGTCGGGTAATTTTTTAATAATTGTTATCCGCTCACAATTG |
| P14 | TGGGATTACACATGGCATG | ||
| xylA of E. coli MG1655 | P15 | ATTCAATTGTGAGCGGATAACAATTATTAAAAAATTACCCGACAAAGGATATACTAAATTATGCAAGCCTATTTTGACC | |
| P16 | TTCTGTTAGTCTGCTTTGTTATTTGTCGAACAGATAATGG | ||
| P17 | ATTCAATTGTGAGCGGATAAC | ||
| xylB of E. coli MG1655 | P18 | GTTCGACAAATAACAAAGCAGACTAACAGAAGGAAGTAACACATGTATATCGGGATAGATCTTG | |
| P19 | TTTCCTTCGTTTGCCCTTTACGCCATTAATGGCAG | ||
| P20 | GTTCGACAAATAACAAAGCAGAC | ||
| rpi of E. coli MG1655 | P21 | ATTAATGGCGTAAAGGGCAAACGAAGGAAACGACAGGAAGTACATATATGACGCAGGATGAATTG | |
| P22 | GATTATAAAGTTAATTTGCGTCATTTCACAATGGTTTTGAC | ||
| P23 | ATTAATGGCGTAAAGGGC | ||
| yajO of E. coli MG1655 | P24 | CATTGTGAAATGACGCAAATTAACTTTATAATCAAGGAACAGAAACAGATGCAATACAACCCCTTAG | |
| P25 | CTCATCCATGCCATGTGTAATCCCATTATTTAAATCCTACGACAGGATG | ||
| P26 | CATTGTGAAATGACGCA | ||
| pPK15304 (pRL814-pgi) | pRL814 backbone | P27 | AATTGTTATCCGCTCACAATTG |
| P28 | TGGGATTACACATGGCATG | ||
| pgi of E. coli MG1655 | P29 | ATTGTGAGCGGATAACAATTAAAGTCACAATTCTCAAAATCAGAAG | |
| P30 | CCATGCCATGTGTAATCCCACGATGATTAACCGCGCCA | ||
| pPK15303-2 | pPK15303 backbone | P31 | CGGCCTGTTTAGCGCTCGGTCTTGCCTT |
| P32 | GGGCATAAACGTGCCGAGGATGACGATG | ||
| Upstream of mrr of Z. mobilis | P33 | TCCTCGGCACGTTTATGCCCGTGACAAC | |
| P34 | TTCAAAAAGCTATCGAGCCTTTTACTTAAAATAATC | ||
| Downstream of mrr of Z. mobilis | P35 | AGGCTCGATAGCTTTTTGAAAAAGCCGTTTC | |
| P36 | ACCGAGCGCTAAACAGGCCGCTAACCTAG | ||
| pPK15357 | pPK15303-2 with modified RBS of gfp | P37 | AAGATATTAGAAGGAGGTCAAAAATGAGCAAAGGAGAAGAACT |
| P38 | TTTTGACCTCCTTCTAATATCTTGTTAAACTAATTCTAGATGT | ||
| pPK15381 | pPK15357 backbone | P39 | GTGCCGAGGATGACGATG |
| P40 | AGCGCTCGGTCTTGCCTT | ||
| Upstream of hsdSc of Z. mobilis | P41 | CTCATCGTCATCCTCGGCACTTCATTGAGCGCAATCTG | |
| P42 | GGTCAAGGTACAGATCGCTTAGGCGATC | ||
| Downstream of hsdSc of Z. mobilis | P43 | AAGCGATCTGTACCTTGACCATGCAGCTG | |
| P44 | GCAAGGCAAGACCGAGCGCTTTGTGCCTGCACATGCTG | ||
| pPK15472 | Upstream of hsdSp of Z. mobilis | P45 | CTCATCGTCATCCTCGGCACGACGATGGAGCAAAGACAG |
| P46 | TAGTGCTCATTATTCCCTCAGTCCATTAGAC | ||
| Downstream of hsdSp of Z. mobilis | P47 | TGAGGGAATAATGAGCACTACTACCGATATCGTTG | |
| P48 | GCAAGGCAAGACCGAGCGCTATCACTTCCCCCGCCTGAG | ||
| pPK15474 | Upstream of hsdRp of Z. mobilis | P49 | CTCATCGTCATCCTCGGCACATGCGGGCGAACATGCCC |
| P50 | TGGCTATGGGATGCGTGCCAACGACCCC | ||
| Downstream of hsdRp of Z. mobilis | P51 | TGGCACGCATCCCATAGCCATTTCCGATTAC | |
| P52 | GCAAGGCAAGACCGAGCGCTACGATCAGTTGCTCGAAAAAG | ||
| pPK15380 | Upstream of cas3 of Z. mobilis | P53 | TGCATTATGATTTATTCAGAAATGATGGAAAATATTATGCATC |
| P54 | GATCTTCTCCCCGCAACCAATAA | ||
| Downstream of cas3 of Z. mobilis | P55 | TCTGAATAAATCATAATGCAGGCTCGGC | |
| P56 | CTCATCGTCATCCTCGGCACCGTTTATCGGCTTTGCTC | ||
| pPK15536 | pRL814 with HsdSp target sequence | P57 | GAGTTCCCCCGGGGGATCCCTAGTTCT |
| P58 | GCGCTTCCTGCAGGAATTCGATATCAAGCT |