Figure 2. Hemozoin constrains the number and function of memory B cells.

C57BL/6 mice were infected with Δpm4Δbp2 and Δlap parasites.

(A–D) Representative flow plots (A) and quantification (B) of splenic CD19⁺CD38⁺IgD^negCD73⁺CD95^neg MBC and the frequency (C) and number (D) of splenic CD19⁺CD38⁺IgD^negCD73⁺CD80⁺PD-L2⁺ MBC by day 30 p.i.

(E) Representative histograms describing frequency (F) and number (G) of CCR6⁺CD19⁺CD38⁺IgD^neg MBC.

(H) Δpm4Δbp2 and Δlap-immune mice were challenged with virulent Pb-ANKA. Sera were collected before (pre) and 5 days after (post) challenge and MSP1_1–19-specific IgG endpoint titers were quantified. Data (mean ± SD) in (A)–(D) and are representative of three independent experiments with n = 4–5. Data (mean ± SD) in (E)–(H) are the summary data of two independent experiments.