Figure 5.

Biocompatibility evaluation of the acellular scaffolds. Cells were seeded on top of the scaffolds and biocompatibility was evaluated after 2 and 7 days of culture in standard conditions. (a) Cell viability and proliferation profiles obtained by MTT assay. Statistical significance: * p < 0.05. (b) GelMA-based scaffolds’ cytotoxicity profiles measured by LDH assay. (c) Qualitative evaluation of cell viability and proliferation was assessed employing Live/Dead assay and confocal microscopy. Live cells are stained in green fluorescence, while dead cells nuclei are stained in red fluorescence using LIVE/DEAD™ Viability/Cytotoxicity Kit, for mammalian cells (Thermo Fisher Scientific Inc., Waltham, MA, USA.). Scale bar 50 µm.