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. Author manuscript; available in PMC: 2022 Sep 7.
Published in final edited form as: Cell Metab. 2021 Sep 7;33(9):1883–1893.e7. doi: 10.1016/j.cmet.2021.08.003

Figure 2. eIF5AHyp regulates mRNA translation initiation.

Figure 2.

Bone marrow-derived macrophages (BMDMs) from control (combination of DhpsLoxp/Loxp and DhpsLoxp/+) and DhpsΔMyel mice (n=3-10 mice per group) were M1-polarized with LPS + IFN-γ and subjected to flow cytometry and polyribosome profiling. (A) Levels of MIP-1α and (B) IL-1β in media from M1-polarized BMDMs. The mRNA from fractions taken during polyribosome profiling were isolated and quantified for (C) Ccl3 and (D) Il1b; (E) Representative immunoblot of total eIF5A after immunoprecipitation with anti-eIF5AHyp antibody or control IgG in RAW 264.7 cell lysate; (F) Quantification of RNA for Ccl3, Il1b, and Tnfa after RNA immunoprecipitation with anti-eIF5AHyp antibody in RAW 264.7 cell lysate (n=4 independent experiments). *p<0.05 by ANOVA. Data are presented as mean ± SEM.