Figure 3. Dhps deletion diminishes adoption of an M1-like proteomic signature.

Bone marrow-derived macrophages (BMDMs) from control (combination of Dhps^Loxp/Loxp and Dhps^Loxp/+) mice (n=6) and Dhps^ΔMyel mice (n=4) were unpolarized (M0) or M1-polarized with LPS + IFN-γ, and multiplex tandem mass tag (TMT) LC-MS/MS-based proteomics analysis was performed. (A) Schematic of experimental design; (B) Quantification of DHPS levels from proteomics; (C) Heatmaps of significantly differentially regulated proteins (identified by their coding genes) in control cells upon M1 polarization, and those that are differentially regulated in Dhps^ΔMyel macrophages upon M1 polarization. Red boxes highlight key inflammatory molecules discussed in the text; (D) KEGG Pathway Analysis of proteins differentially regulated in Dhps^ΔMyel macrophages upon M1 polarization. *p<0.05 by ANOVA. Data are presented as mean ± SEM.