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. Author manuscript; available in PMC: 2022 Sep 1.
Published in final edited form as: Curr Protoc. 2021 Sep;1(9):e235. doi: 10.1002/cpz1.235

Figure 1.

Figure 1.

Plasmid maps of HPV18 (cloned in pBR322) and selectable marker plasmid (pRSVneo). The HPV genome is cloned in pBR322, a bacterial plasmid vector, for propagation in bacteria. A restriction digest (the EcoRl site is highlighted in red to release the HPV18 genome from pBR322 vector) releases the HPV genome from this vector for HPV genome recircularization (Support Protocol 2) prior to transfection (Basic Protocol 1). The pRSVneo plasmid is co-transfected with the recircularized HPV genome and enables transient G418 selection of transfected cells (Basic Protocol 1).