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. Author manuscript; available in PMC: 2022 Sep 1.
Published in final edited form as: Curr Protoc. 2021 Sep;1(9):e235. doi: 10.1002/cpz1.235

Figure 3.

Figure 3.

HPV keratinocyte colonies stained with methylene blue 18 days after electroporation. HFK colonies resulting from the method described in Basic Protocol 1 were stained with methylene blue as described in Basic Protocol 2 and imaged on a Syngene white box imager. Comparing the number of colonies formed by transfecting with HPV16 and pRSVneo versus pRSVneo and pUC19, can help determine whether the concentration of G418 used in selection should be increased or decreased. A plate of control plasmid(1.g.pUC19) transfected keratinocytes can help determine the effectiveness of the G418 drug used.