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. Author manuscript; available in PMC: 2022 Sep 1.
Published in final edited form as: Curr Protoc. 2021 Sep;1(9):e235. doi: 10.1002/cpz1.235

Table 1.

Commonly used HPV plasmid genome DNAs and enzymes used for recircularization.

HPV Plasmid name Bacterial Plasmid Vector Restriction enzyme and buffer used for recircularization Reference
16 pEFHPV-16W12E pUC BamHl (NEB R0136L); 10X CutSmart Buffer (NEB B7204S) (Flores et al., 1999)
18 pBR322-HPV18 pBR322 EcoRl (NEB R0101L); 10X Buffer 2.1 (NEB B7202S) (Boshart et al., 1984)
31 pBR322-HPV31 pBR322 EcoRl (NEB R0101L); 10X Buffer 2.1 (NEB B7202S) (Frattini, Lim, & Laimins, 1996)