Official websites use .gov
A
.gov website belongs to an official
government organization in the United States.
Secure .gov websites use HTTPS
A lock (
) or https:// means you've safely
connected to the .gov website. Share sensitive
information only on official, secure websites.
Recipe for hybridization buffer (Basic Protocol 3). Small blots (11×14cm) each require 10ml of hybridization buffer while larger blots (15×20cm) require 20mls. It is important to add the buffer constituents in the order shown, otherwise they can precipitate.
| Stock/Total Volume | 10ml | 20ml | 30ml | 50ml | 100ml |
|---|---|---|---|---|---|
| 20X SSC | 1.5ml | 3ml | 4.5ml | 7.5ml | 15ml |
| H2O | 6.3ml | 12.6ml | 18.9ml | 31.5ml | 63ml |
| 50X Denhardts | 1ml | 2ml | 3ml | 5ml | 10ml |
| 20% SDS | 1ml | 2ml | 3ml | 5ml | 10ml |
| 10mg/ml Salmon sperm DNA | 0.2ml | 0.4ml | 0.6ml | 1ml | 2ml |
