Skip to main content
. Author manuscript; available in PMC: 2021 Sep 10.
Published in final edited form as: J Steroid Biochem Mol Biol. 2020 Apr 30;201:105688. doi: 10.1016/j.jsbmb.2020.105688

Fig 1. HA expression in sorted Hs578T subclones.

Fig 1.

A. Parental Hs578T were sorted with biotinylated hyaluronic acid binding protein (bHABP) into HAHigh and HALow populations. The sorted cells were expanded in culture and re-sorted up to six times. Top: Phase images of parental Hs578T. Bottom: Flow cytometry data of bHABP binding to cell-associated HA detected by Alexa Fluor 488 streptavidin of parental Hs578T highlighting the HAHigh and HALow sorted regions. Isotype control, dashed line. B. Top Panels: Phase images of HAHigh and HALow subclones. Bottom Panels: Flow cytometry data of bHABP binding to cell-associated HA of HAHigh and HALow subpopulations after 6 rounds of cell sorting. Isotype control, dashed line. C. Validation of the effectiveness of the sort into high and low HA subpopulations. Left: Confocal imaging of fluorescently labeled bHABP binding to cell-associated HA on Hs578T subclones. Right: Phase images and visualization of pericellular HA coat by exclusion of sedimenting fixed erythrocytes on Hs578T subclones.