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. 2021 Aug 30;17(8):e1009786. doi: 10.1371/journal.pgen.1009786

Fig 6. Prl8a2 was under the control of canonical Notch signaling.

Fig 6

The relative mRNA level of Prl and Prl8a2 was examined in Rbpj f/f versus Rbpj d/d decidua (A) and DNMAML tg/+ versus DNMAML tg/+;PR-cre decidua (B). (C) GFP signals in implantation sites of mice carrying Rbpj responsive element-driven GFP on day 6 and 8. Nuclei were stained by DAPI. pdz, primary decidual zone; sdz, secondary decidual zone. Scale bar: 50μm and 100μm. (D) Chromatin immunoprecipitation assay showed the direct binding of Rbpj on Prl8a2 promoter. IgG was served as isotype control. Gapdh was served as negative control. (E) Luciferase analysis revealed Prl8a2 promoter activity in the presence of DAPT or DMSO. (F) Prl8a2 promoter activity was enhanced by NICD and RBPJ, which was reversed by DNMAML. Data in (A), (B) (D) and (E) are presented as mean±SEM. **p<0.01, ***p<0.001, a vs b and b vs c: P < 0.01, a vs c: P < 0.001.