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. 2021 Sep 10;16(9):e0256655. doi: 10.1371/journal.pone.0256655

Fig 7. ATF4- and CCK-positive neurons in the corticolimbic system.

Fig 7

Representative immunofluorescent images of 8-week-old OLETF and LETO rats in the anterior cingulate cortex (ACC; A, B, C, and D), basolateral amygdala (BLA; E, F, G, and H), and hippocampal cornu ammonis area 3 (CA3; I, J, K, and L). Labeling for activating transcription factor 4 (ATF4; A, E, and I) and cholecystokinin (CCK; B, F, and J) and merged images (C, G, and K for OLETF rats; D, H, and L for LETO rats). Arrows or arrow heads indicate neurons expressing ATF4 and CCK or only CCK, respectively. Scale bars = 100 μm. Rad: radiatum layer; Py: pyramidal cell layer; Or: oriens layer. The percentages of neurons co-expressing ATF4 and CCK in the ACC, prelimbic cortex (PL), infralimbic cortex (IL), lateral amygdala (LA), BLA, CA1, CA2, CA3, and dentate gyrus (DG; M). ATF4+: ATF4-positive; CCK+: CCK-positive. Values represent mean ± standard error of means. * Significant difference from age-matched LETO rats, p < 0.05; † significant difference from same strain at 8 weeks of age, p < 0.01, two-way ANOVA.