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. 2021 Sep 10;17(9):e1009785. doi: 10.1371/journal.pgen.1009785

Fig 1. MED30 is essential for early cardiac development.

Fig 1

(A) Whole embryonic morphology of constitutive cardiomyocyte-specific knockout (cKO) and control (Ctrl) littermates at E11.5 and E12.5. Arrow: edema chest; Asterisk: hemorrhage. Scale bar: 1 mm. n = 3. (B) Cardiac morphology (Top and middle) and H&E images (Bottom) of Med30 cKO embryos and Ctrl littermates at E11.5. Arrow: edema chest. Scale bar: 100 μm. n = 3. (C) Quantitation of compact zone thickness in Med30 cKO and Ctrl hearts at E10.5 and E11.5. RV: right ventricle; LV: left ventricle. n = 3. (D) Representative immunostaining images of EdU-labeled (red) heart sections from Med30 cKO and Ctrl mice at E9.5 and E10.5, using an antibody against NKX2.5 as cardiomyocyte marker (green). DNA is stained with DAPI (blue). Arrow: Edu-positive cardiomyocytes. Scale bar: 10 μM. (E-F) Quantification (%) of Edu-positive cardiomyocytes (CM) in (E) E9.5 and (F) E10.5 Ctrl (blue) vs. Med30 cKO (red) heart areas as indicated. Com: compact zone; Tra: trabeculae. n = 3. Data are represented as the mean ± SEM. *P < 0.05, by 2-tailed Student’s t test.