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. 2021 Sep 10;17(9):e1009785. doi: 10.1371/journal.pgen.1009785

Fig 2. Loss of MED30 results in degradation of Mediator core in developing cardiomyocytes.

Fig 2

(A-B) Representative immunoblots (A) and quantification analysis (B) of MED30 and MED Tail submodule subunits MED15, MED16, MED23, MED24 and MED29; MED Middle submodule subunits MED1, MED4, MED31 and MED14; MED Head submodule subunits MED6, MED8, MED17, MED18 and MED20, and Kinase module subunits MED12 and MED13 in hearts isolated from Med30 cKO and control (Ctrl) embryos at E10.5. GAPDH served as a loading control. n = 3. (C-D) Co-Immunoprecipitation (Co-IP) of E10.5 heart lysates with antibody to MED4 (C) or MED12 (D), followed by Western blot (WB) analysis for MED subunits. 40 hearts from each genotypes were pooled for one IP experiment. (E) Heatmap representation of transcript levels of selected Mediator subunits in Med30 cKO and Ctrl hears at E10.5. Data are represented as the mean ± SEM. *P < 0.05, by 2-tailed Student’s t test.