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. 2021 Sep 10;17(9):e1009901. doi: 10.1371/journal.ppat.1009901

Fig 1. Neddylation promotes RNA virus-induced IFN-α production in myeloid cells.

Fig 1

(A-F, I, J) BMDMs or mDCs were cultured from the indicated mouse models. After immunoblotting (IB) analysis with the indicated antibodies to confirm the deficiency of UBA3 (A, I) or NEDD8 (E), these myeloid cells were infected with the indicated RNA viruses for the indicated time periods. Then the supernatants were subjected to ELISA (B, C, F, J). The survival of UBA3-deficient BMDMs before and 24 h after RNA virus infection was measured by ATP lite assays (D). (G) IB analysis to confirm the efficiency of MLN4924 pretreatment (0.1 μM, 3 h) in wild type (WT) BMDMs. (H) After MLN4924 pretreatment (0.1 μM, 3 h), WT BMDMs were infected with SeV for the indicated time periods. Then the supernatants were subjected to ELISA. Quantitative data are shown as Mean ± SD (n = 3 per group). *p< 0.05; **p< 0.01; ***p< 0.001; NS, not significant. CUL-N8, neddylated Cullin 1.