Fig 7. The neddylation of mammalian IRF7 prevents its dimerization with IRF5.
(A-C) After BMDMs were cultured from Nedd8F/F and Nedd8ΔMye mice or 24 h after HEK-293T cells were transfected with the indicated mammalian expression vectors, the cells were pretreated with or without MLN4924 (0.5 μM, 3 h), followed by SeV infection for various periods of time as indicated. Then the phosphorylation and expression of murine IRF7 was detected by IB with the indicated antibodies. P-IRF7, phosphorylated IRF7 at Ser437/438. (D, E) Twenty-four hours after HEK-293T cells were transfected with the indicated mammalian expression vectors, the cells were infected with SeV for 6 h or left untreated. Then the interaction between exogenous murine IRF7 and exogenous human IRF3 (D) or exogenous human IRF5 (E) was determined by Co-IP.