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. 2021 Sep 10;17(9):e1009884. doi: 10.1371/journal.ppat.1009884

Fig 3. WT and variant candidalysin peptides similarly induce damage, cytokine response, and membrane leakage.

Fig 3

(A) In silico modeling of candidalysin from both SC5314 and 529L reveal dihelical peptides with relatively conserved structure. (B) Sulforhodamine B (SRB) loaded lipid vesicles were treated with WT and VAR candidalysin for 1 h and fluorescence intensity (ex. 550/em. 590 nm) used to assess the capacity to induce membrane leakage (mean ± SD). A431 vaginal epithelial cells were challenged with wild-type (WT) or variant (VAR) candidalysin at doses of 70, 35, 15, and 3 μM and resulting (C) IL-1β and (D) LDH responses assessed (mean ± SD). Values were blank subtracted using vehicle-treated controls. A431 experiments were conducted as described above but candidalysins (15 μM) were added to buffered cell culture media adjusted to the indicated pH and (E) IL-1β (mean ± SD) and (F) LDH (mean ± SD) measured. All experiments were conducted in biological triplicate. Data was analyzed using multiple t-test.