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. 2021 Sep 10;17(9):e1009884. doi: 10.1371/journal.ppat.1009884

Fig 7. Introduction of the SC5314 ECE1 locus in isolate 529L partially restores pathogenicity.

Fig 7

Mice were challenged with strain 529L harboring SC5314 ECE1 (529L-WO) or an additional copy of 529L ECE1 (529L-VO). Mice (n = 12 per group) underwent vaginal lavage at d 3 post-infection and lavage fluids assessed for (A) CFU by microbiological plating (median ± IQR), (B) PMN recruitment by microscopy (median ± IQR), (C) IL-1β by ELISA (median ± IQR), and (D) tissue damage by LDH assay (median ± IQR). Statistical significance was assessed using Mann-Whitney U test. A431 vaginal epithelial cells were challenged with these same strains, along with isolate 529L, and (E) IL-1β measured by ELISA (mean ± SD) and (F) LDH release (mean ± SD) assessed. Statistical significance was assessed using one-way ANOVA and Dunnet’s post-test. All in vitro experiments were conducted in biological triplicate. *, p < 0.05, ** p < 0.01, *** p < 0.001.