Fig 4.

Production of pyocyanin (A), rhamnolipids (B) and MVs (C) are reduced in P. aeruginosa tat mutants. (A) Pyocyanin levels are shown in the P. aeruginosa wild type, ΔpqsA, tatA, and tatABC deletion mutants and the tatA mutant complemented with plasmid-borne tatA. The impact of exogenous PQS (40 μM) on the tatA and ΔtatABC mutants is also shown. Insert panel shows the absence of green pigment in the tatA mutant compared with the wild type and complemented tatA mutant. (B) Rhamnolipid production in the tatA and ΔtatABC mutants compared with the wild type, ΔpqsA mutant and tatA complemented with plasmid-borne tatA. (C) Comparison of MV production in the tatA mutant, complemented tatA mutant and in a double tatA ΔpqsA mutant compared with the wild type strain. Experiments were repeated in triplicate at least twice. ***p < 0.001, **p < 0.01; n.s. not significant.