Table 2. Sequence alignment analysis for genetic differences within the viral genomes from isolated 19A Korean (EPI_ISL_426163), 20A Italian (EPI_ISL_514432-S66), and 20I/501Y.V1 (B.1.1.7) British UK (EPI_ISL_736997) SARS-CoV-2 strains.
The missense mutations among the SARS-CoV-2 strains used in this study are listed (genomic and amino acid substitutions, and amino acid substitutions according to the nomenclature of the ORF1ab peptides).
| Viral genome |
Genomic
substitution |
Amino acid
substitution |
Amino acid
substitution (ORF1ab peptides) |
|
EPI_ISL_426163
(Korea) |
C17104T |
ORF1ab: H5614Y |
Helicase: H290Y |
| C18167T |
ORF1ab: P5968L |
Exonuclease: P43L |
|
| G26167T | ORF3a: V259L | ORF3a: V259L | |
| T28144C | ORF8: L84S | ORF8: L84S | |
|
EPI_ISL_514432
(Italy) |
C3927T |
ORF1ab: S1221L |
nsp3: S403L |
| C14408T | ORF1ab: P4715L | RdRp: P323L | |
| C22458T | S: T299I | S: T299I | |
| A23403G | S: D614G | S: D614G | |
|
EPI_ISL_736997
(United Kingdom) |
A1752G | ORF1ab: Y496C | nsp2: Y316C |
| C3267T | ORF1ab: T1001I | nsp3: T183I | |
| C5388A |
ORF1ab: A1708D |
nsp3: A890D | |
| C6636T | ORF1ab: T2124I | nsp3: T1306I | |
| T6954C | ORF1ab: I2230T | nsp3: I1412T | |
| C14408T | ORF1ab: P4715L | RdRp: P323L | |
| A23063T | S: N501Y | S: N501Y | |
| C23271A | S: A570D | S: A570D | |
| A23403G | S: D614G | S: D614G | |
| C23604A | S: P681H | S: P681H | |
| C23709T | S: T716I | S: T716I | |
| T24506G | S: S982A | S: S982A | |
| G24914C | S: D1118H | S: D1118H | |
| C27972T | ORF8: Q27stop | ORF8: Q27stop | |
| G28048T | ORF8: R52I | ORF8: R52I | |
| A28111G | ORF8: Y73C | ORF8: Y73C | |
| G28280C | N: D3L | N: D3L | |
| G28881A | N: R203K | N: R203K | |
| G28882A | N: G204R | N: G204R | |
| C28977T | N: S235F | N: S235F |