Figure 2.
Muscle contains two capillary EC populations characterized by distinct Atf3/4
(A) Left: heatmap with log fold changes of 50 top marker genes of CapEC 1 relative to CapEC 2. Right: violin plot showing the distribution of Atf3 and Atf4 expression within different mEC populations.
(B) Representative fluorescence images showing ATF3 (gray) and CD31 (red) combined with type I (MHCI, green), type IIa (MHCIIa, blue), and type IIb (MHCIIb, yellow) fiber-type staining on cross-sections in oxidative and glycolytic areas of GAS. Arrows indicate ATF3+CD31+ ECs. Scale bar, 50 μm.
(C) Quantification of the percentage of ATF3+ vessels in oxidative and glycolytic areas of GAS (n = 6).
(D) Representative fluorescence images showing ATF3 (gray) and CD31 (red) combined with type I (MHCI, green), type IIa (MHCIIa, blue), and type IIb (MHCIIb, yellow) fiber-type staining on thick longitudinal sections of GAS. Arrows indicate ATF3+CD31+ ECs. Scale bar, 20 μm.
(E) Representative images of CD31 (red), ATF3 (green), and Hoechst (blue) staining of RmECs and WmECs that were cultured for 7 days. Scale bar, 50 μm.
(F) Quantification of the percentage of ATF3high ECs in RmECs versus WmECs (n = 3).
(G) Heatmap of the top 75 most highly variable genes in RmECs versus WmECs.
(H) TF motif enrichment analysis of upregulated genes in RmECs over WmECs.
(I) ATF4-dependent biological process analysis of upregulated genes in RmECs over WmECs.
Two-tailed unpaired Student’s t test in (C) and (F) (∗p < 0.05). Bar graphs represent mean ± SEM. See also Figure S2 and Data S1.