Figure 1.

Characterization of circFOXP1 in RCC. (a) The exonic information about circFOXP1 was elucidated using the circBase dataset (http://www.circbase.org/). (b) Expression level of circFOXP1 in thirty pairs of RCC tissue samples was measured. (c) Expression level of circFOXP1 in RCC cell lines and normal renal cell HK-2 was tested. (d, e) Random hexamer primers were applied, and the results were analyzed using qRT-PCR in ACHN (d) and 786-O cells (e). (f, g) ACHN (f) and 786-O (g) cells were under actinomycin D treatment; relative circFOXP1 and liner FOXP1 (m-FOXP1) expression was detected using qRT-PCR. (h, i) Expression levels of circFOXP1 in the cytoplasm or nucleus of ACHN (h) or 786-O (i) cells were detected by qRT-PCR after cellular RNA fractionation. All experiments were repeated in triplicate, ^∗∗P < 0.01 and ^∗∗∗P < 0.001.