Figure 4.

BCL2 gene is the common target of miR-361-3p and miR-324-5p

StarBase predicts miR-361-3p and miR-324-5p target on BCL2 (A), transfected into HEK293T cells (B). RIP-quantitative real-time PCR experiments confirm the interaction between miR-361-3p and miR-324-5p and BCL2 mRNA and protein expression level (C), quantitative real-time PCR, and western blot experiments detect BCL2 expression in U251, U87 cells treated with sh-NC, sh-circ#1, sh-circ#2 (D), and inhibitors of miR-361-3p and miR-324-5p (E). ∗∗p < 0.01.