Figure 5.

Antitumor efficacy of CAR-T cells in vivo

(A) Serial bioluminescence imaging of NSG mice injected subcutaneously with A549^luc cells on the left flank and A549^luc-CXCL13 cells on the right flank. 10 days after tumor engraftment, the mice were injected with 5 × 10⁵ CAR⁺ T cells as indicated. n = 5 mice per group. Error bars represent mean ± SD for each time point (n = 5). (B) The tumor volume of the left tumors (A549^luc) and right tumors (A549^luc-CXCL13) over 28 days was measured. Error bars represent mean ± SD for each time point (n = 5). (C) The copy number of CAR gene in the left and right tumor tissues was analyzed. ∗∗p < 0.01.