Figure 4.

Cav-1 depletion reduces sensitivity to SSH20 in vitro

(A and B) Cell cytotoxicity was determined in MP2-shCtrl/shCav-1 and H23-shCtrl/shCav-1 stable cells with different doses of SN-38 and SSH20 for different periods of drug exposure (6, 12, 72 h), using alamarBlue assay. ∗∗∗p < 0.001; ∗∗p < 0.01; ∗p < 0.05. (C) Half-maximal inhibitory concentration (IC₅₀) of each treatment group was calculated and compared in MP2-shCtrl/shCav-1 and H23-shCtrl/shCav-1 cells. Fold change (IC₅₀ shCav-1/IC₅₀ shCtrl) shows increasing fold-change difference with shorter time periods of exposure. (D) SSH20-induced apoptosis was confirmed by immunoblotting for cleaved PARP (cPARP) and cleaved caspase 3 (cCas3). GAPDH shown as equal loading control.