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. 2021 Sep 10;10(9):60. doi: 10.1038/s41389-021-00350-x

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 2 — A Western blotting analysis of TRIM3 levels in the indicated breast cancer cell lines stably overexpressing TRIM3 and silencing for TRIM3. Cell viability was measured using MTT (B) and colony formation (C) assays in the indicated cell lines treated with E2 (10 nM) and TAM (1 μM). Statistical data are presented as the mean ± SD. D Soft agar assay of the indicated cells treated with E2 (10 nM) and TAM (1 μM) (left panel) and the statistical significance of colony formation was assessed in the right panel. Data are presented as the mean ± SD. P < 0.05. E Representative images of the tumors in the xenografts (left panel). Tumor growth curves (middle panel) and tumor weight (right panel) of the indicated xenograft tumors (n = 6/group). Data are presented as the mean ± SD. *P < 0.05, **P < 0.01.