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. 2021 Sep 10;12:5373. doi: 10.1038/s41467-021-25710-4

Fig. 2. TNF-α accelerates the directional migration of AS-MSC in vivo.

Fig. 2

a Schematic diagram of the in vivo migration assay. MSC were transplanted into nude mice, and TNF-α was injected around the MSC. In vivo imaging and an immunohistochemical (IHC) assay were then performed. b The bioluminescence area of MSC increased from day 0 to day 5, and the bioluminescence area of AS-MSC (n = 9) was larger than that of HC-MSC (n = 9) on days 3 (P = 6.6784E−8) and 5 (P = 1.9895E−14) after injection. c The number of migratory AS-MSC determined by counting cells per field (n = 20) was higher than that of migratory HC-MSC (P = 7.1465E−7). Data were analyzed using two-tailed Student’s t-test. Values are presented as the mean ± SD. *** Indicates P < 0.001. Scar bar = 100 μm.