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. 2020 Sep 7;6:70. doi: 10.1038/s41378-020-0180-0

Fig. 2. Active generation of water-water-oil double emulsions.

Fig. 2

a Schematic of the active method to generate water-water-oil double emulsions in a pico-injection channel and image of the double emulsions generated at flow rates of 200 μL/h (DEX-rich), 600 μL/h (PEG-rich) and 3000 μL/h (oil). Scale bar: 100 μm. b A plot of the core-droplet diameter and generation frequency as a function of the oil flow rate, which is varied from 500 μL/h to 10,000 μL/h. c Image of the dual-injector device for successive injection of the DEX-rich phases with and without fluorescence. Scale bar: 100 μm. d Fluorescence microscopy images of the resultant double emulsions at different flow-rate ratios of the two injections. The flow rates of PEG-rich and oil phases are set constant at 600 and 3000 μL/h, while the flow rates of the DEX-rich phases with and without fluorescence are coupled at 50:350, 100:300, 200:200, 300:100 and 350:50 μL/h, respectively. Scale bar: 100 μm