Skip to main content
. Author manuscript; available in PMC: 2022 Nov 1.
Published in final edited form as: Thromb Haemost. 2021 Mar 11;121(11):1448–1463. doi: 10.1055/s-0041-1726093

Figure 4. Mac-1 and PAR1, but not EPCR, are required for APC inhibition of extracellular traps in macrophages.

Figure 4.

J774A.1 macrophages were pretreated with PAR1, Mac-1 or EPCR function-blocking antibodies (15–20 μg/mL for 1h) followed by treatment with APC (20 nM for 3h) before stimulation with poly(I:C) (10 μg/mL for 4h) (APC remained in the media after addition of poly(I:C)). Cells were fixed and permeabilized, followed by staining for histone H3 with mouse anti-histone H3 antibody and Alexa Fluor 555-conjugated goat anti-mouse IgG. DNA was stained with Sytox Green. Immunofluorescence images were taken by confocal microscopy. Arrows indicate cells with extracellular trap formation. The inset boxes from each group are magnified. Dot plot shows quantification of ET-forming cells. Experiments were repeated at least three times. **p < 0.01. Ctrl, control. Scale bar: 10 μm.