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. Author manuscript; available in PMC: 2022 Nov 1.
Published in final edited form as: Thromb Haemost. 2021 Mar 11;121(11):1448–1463. doi: 10.1055/s-0041-1726093

Figure 6. APC inhibits poly(I:C)-induced extracellular traps in human neutrophils.

Figure 6.

Human blood neutrophils were pretreated with APC (A) or a signaling-selective mutant of APC (APC-2Cys) (20 nM for 3h) (B), followed by stimulation with poly(I:C) (10 μg/mL for 4h) (APC remained in the media after addition of poly(I:C)). Cells were then fixed, permeabilized and Myeloperoxidase (MPO) was stained with rabbit anti-MPO antibody and Alexa Fluor 555-conjugated goat anti-rabbit IgG. DNA was stained with Sytox Green. Immunofluorescence images were taken by confocal microscopy. Arrows indicate cells with extracellular trap formation. The inset boxes from each group are magnified. Dot plot shows quantitation of NET-forming cells in (A) or (B). All experiments were repeated at least three times. **p < 0.01. Ctrl, control. Scale bar: 20 μm.