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. 2020 Jun 15;6:28. doi: 10.1038/s41378-020-0143-5

Fig. 4. Optimization of enrichment time and enrichment cycle number for immunoassays utilizing the hybrid PnP microfluidic device.

Fig. 4

Enrichment time refers to the incubation time allowing for the analytes to be adsorbed on paper in a static status between each cycle of pumping through paper. a The relationship between the enrichment time and net brightness difference between the analyte and the negative control. b The relationship between the number of enrichment cycles and net brightness difference between the analyte and the negative control.