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. 2021 Sep 10;135(17):2127–2142. doi: 10.1042/CS20210644

Figure 4. EphB2-KO mice have reduced tubular α-SMA staining and renal protein levels of fibrotic markers following IR injury.

Figure 4

(A) Frozen kidney sections from EphB2+/+ and EphB2−/− mice following IR injury were stained with α-SMA (red) and DAPI/DNA (blue) and analyzed using confocal microscopy. Magnification, ×200. (B) The graphs summarized the results of α-SMA deposition quantified by ImageJ. *P<0.05 vs. EphB2+/+ contralateral kidney (CTL). (C) Western blots of FN, α-SMA, N-cadherin, and GAPDH from CTL and IR-injured kidneys of EphB2+/+ and EphB2−/− mice. Molecular weight was labeled on the right. n=5 animals/group. (D–F) The graphs summarized the results of band density measurements for FN (D), α-SMA (E), and N-cadherin (F). *P<0.05 vs. EphB2+/+ CTL. #P<0.05 vs. EphB2+/+ IR-injured kidney.