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. 2021 Sep 10;12:5376. doi: 10.1038/s41467-021-25715-z

Fig. 1. Kinetic of FV infection and effector phenotype of NK cells upon FV infection.

Fig. 1

C57BL/6 mice were infected with FV for 3, 7, 12 and 28 days. Single-cell suspensions from spleens and bone marrow (BM) were prepared and used for the analysis of viral loads via Infectious Center assay (a). At least six mice from at least two individual experiments were used for the analysis. b Single-cell suspensions were stained for NK cell markers (CD3− NK1.1+ CD49b+) and analysed for the activation by measuring the early activation marker CD69. Naive mice were used as control. Mean values ± SEM were indicated by circles (bone marrow) and rectangles (spleen). Statistically significant differences between bone marrow and spleen (a) and CD69+ NK cells (b) were analysed by Mann–Whitney test. At 7 dpi, splenocytes were stained for NK cell markers and CD27 and CD11b (c). The effector phenotype of splenic NK cells (e) and NK cells from the bone marrow (d), KI-67, FasL, TNF, IFNγ and GzmB is displayed as spider plots. Data of NK cells from naive mice were displayed in blue and from FV infection in red. Statistically significant differences were analysed between naive and FV groups with an unpaired t-test (CD27 CD11b, KI-67, FasL, TNF (%), IFNγ (%)) or Mann–Whitney test (TNF (MFI), IFNγ (MFI)) within the bone marrow or spleens. A minimum of six mice from two independent experiments was used for the analysis. Significances are indicated as follows: *p < 0.05, **p < 0.01, ***p < 0.001. Applied statistical tests were two-sided. Source data are provided as a Source Data file. ns not significant.