Figure 4.

Wildtype and Il6^−/− mice demonstrate similarly increased macrophage numbers. (A) CD45⁺ cells were identified from live and singlet cells. (B) Lineage (Lin) gate was used to exclude neutrophils (Ly6G), eosinophils (SiglecF), NK cells (NK1.1), T cells (CD4, CD8), and B cells (B220), and gate forward CD11b⁺Lin⁻ mononuclear phagocytes. (C) Quantitative CD45 expression was used to separate CD45^dim from CD45^high cells. (D) Microglia were defined as CD45^dimCD64⁺MHCII^low from wildtype and Il6^−/− mice both with and without laser. (E): MHCII⁻ macrophages (macs) were delineated as CD45^highCD64⁺MHCII⁻, and CD45^highMHCII⁺ cells were gated forward. (F): CD11c⁻ and CD11c⁺ macrophages were identified by CD64⁺MHCII⁺CD11c⁻ and CD64⁺MHCII⁺CD11c⁺, respectively. Dendritic cells (DC) were designated as CD64⁻MHCII⁺CD11c⁺. (G) Microglia were not changed by genotype or laser. (H–J): MHCII⁻, CD11c⁻, and CD11c⁺ macrophages were equally increased by laser in wildtype (blue) and Il6^−/− (red) mice (* = p < 0.05, ** = p < 0.01, *** = p < 0.001, N = 5–9 per group). (K) Dendritic cells were also increased by laser in both wildtype and Il6^−/− mice.