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. 2020 Oct 19;6:93. doi: 10.1038/s41378-020-00201-6

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 2 — a Micrographs showing microwell areas from a microfluidic device with spheroids after 7 and 14 days of culture (scale bar = 250 µm). The insert shows a zoomed-in image of a single microwell/spheroid (scale bar = 100 µm). The right image shows live/dead staining at day 14 (live (green), dead (red fluorescence)). b Viability of spheroids from the six PDX lines after 14 days of culture were tested in this study to compare microfluidic (gray bars) versus standard (black bars) culture methods. Live/dead staining was used for quantification (p < 0.05, n = 6). c Increase in spheroid diameter was observed after 14 days of culture (p < 0.05, n = 6)