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. 2020 Oct 19;6:93. doi: 10.1038/s41378-020-00201-6

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 3 — a Micrographs showing cells from three different PDX lines cultured on Matrigel and in microfluidic microwells. Note the difference the uniform spheroid formation in microfluidic microwells with the variable spheroid sizes on Matrigel. No spheroids could be formed for PDX line PH592 on Matrigel. Scale bar = 250 µm. b Viability for cultures from all PDX lines at day 14 comparing microfluidic (gray bar) and Matrigel (black bar) culture conditions. Microfluidic cultures showed significantly better viability (p < 0.05, n = 6) for all PDX lines except for PH704