Fig. 4. Phenotype assessment of OC spheroids.
a A representative spheroid from a microfluidic device stained for an epithelial marker EpCAM and a proliferation marker Ki-67 as well as the nuclear dye DAPI. b PCR-RT results for the proliferation marker Ki-67 and epithelial markers PAX8, CK-7, and EpCAM. The results show similar or higher expression levels in microfluidic cultures compared to the standard culture method (p < 0.05, n = 3)