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. 2020 Oct 19;6:93. doi: 10.1038/s41378-020-00201-6

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 5 — a, b Pictures showing a microfluidic device in serial perfusion (a) and parallel testing (b) modes. In serial perfusion, the device is configured to connect culture chambers serially. All culture chambers in (a) are filled with blue dye. In parallel perfusion, the device is reconfigured to sequester individual culture compartments and infuse them with dyes of different colors (blue, yellow, green, and red). c–e Images showing spheroids formed after seeding 25,000 (c), 50,000 (d), and 100,000 (e) cells into the device. Scale bar: 300 µm. f A graph showing spheroid diameter as a function of inoculation concentration for the 8 chambers of the device. Chamber 1 is closest to the inlet used for cell seeding, and chamber 8 is furthest. Spheroids were measured 24 h after seeding (n = 8)