Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2021 May 24;7:38. doi: 10.1038/s41378-021-00267-w

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Fig. 1 — a The workflow of the DEPMiC. Culture medium containing cells, oil-surfactant mixture, and culture medium containing Pluronic F-127 were introduced to the emulsion generator. The generated double emulsions (DEs) are then transferred onto the emulsion trapper, where the geometry of the microwells was tailored for the produced DEs. The spheroids were observed to be unperturbed upon subsequent treatment with the DEs, such as oil layer removal, fluorescent staining, and drug treatment. The in situ analysis of individual spheroids is made possible by a customized fluorescence measurement platform. b A representative microscopic image (left) and size distribution of the produced DEs (right) demonstrate that the produced DEs are highly uniform with a diameter of ~150 μm. The dashed line on the histogram represents a Gaussian fit of the raw data. c A representative microscopic image showing that the emulsion trapper treated with BSA is capable of localizing the produced DEs. Scale bars: 200 μm.