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. 2020 Jun 1;6:44. doi: 10.1038/s41378-020-0153-3

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 2 — a Schematics of the in vitro experimental setup for the characterization of spatial temperature distribution. b Normalized distribution of the temperature increase along the y axis (x = 200 µm). c Relative rise in temperature as a function of distance between the tips of the immersed optrode and the external temperature sensor. d Calibration curves: optical power vs. rise in temperature. e 2D representation of the distribution of temperature rise along two perpendicular axes x and y. The location of the probe tip is considered the origin of the coordinate system