Table 1. Primer sequences of miRNAs and mRNAs for RT-qPCR.
Gene | Forward (5′–3′) | Reverse (5′–3′) |
---|---|---|
mmu-miR-1a-3p | GGGGCTGGAATGTAAAGAAGT | GTGCGTGTCGTGGAGTCG |
mmu-miR-133a-3p | GGTTTGGTCCCCTTCA | GTGCGTGTCGTGGAGTCG |
mmu-miR-204-5p | TCCCTTCCCTTTGTCATCCT | GTGCGTGTCGTGGAGTCG |
mmu-miR-7a-5p | GGGGGTGGAAGACTAGTGATTT | GTGCGTGTCGTGGAGTCG |
mmu-miR-10a-5p | GGCAACCCTGTAGATCCGAA | GTGCGTGTCGTGGAGTCG |
mmu-miR-341-3p | GGGTTCGGTCGATCGGT | GTGCGTGTCGTGGAGTCG |
U6 | CTCGCTTCGGCAGCACATATACT | ACGCTTCACGAATTTGCGTGTC |
IL-1β | GAAATGCCACCTTTTGACAGTG | TGGATGCTCTCATCAGGACAG |
IL-6 | CCAGAAACCGCTATGAAGTTCCT | CACCAGCATCAGTCCCAAGA |
GAPDH | GCCAAGGCTGTGGGCAAGGT | TCTCCAGGCGGCACGCAGA |