KNU represses both CLV3 and WUS in the FM. (A) Wild-type flower. (B) pCLV3:KNU flower. (C and D) Expression patterns of CLV3 in stage 3 flower buds of pCLV3:GFP-ER (C) and pCLV3:KNU pCLV3:GFP-ER (D). (E and F) Expression of WUS in stage 3 flower buds of pWUS:WUS-linker-GFP (E) and pCLV3:KNU pWUS:WUS-linker-GFP (F). (G) pCLV3:KNU-NLS flower. (H and I) Expression of CLV3 (H) and WUS (I) in stage 3 flower buds of pCLV3:KNU-NLS pCLV3:GFP-ER and pCLV3:KNU-NLS pWUS:WUS-linker-GFP. (J and K) GFP signal in the SAM (J) and stage 3 flower buds (K) of pCLV3:GFP-ER. (L and M) GFP signals in stage 3 flower buds of pCLV3:KNU-GFP (L) and pCLV3:KNU-GFP-NLS (M). (N) pWUS:KNU flower. (O and P) Expression of CLV3 (O) and WUS (P) in stage 3 flower buds from pWUS:KNU pCLV3:GFP-ER and pWUS:KNU pWUS:WUS-linker-GFP (Scale bars, 1 mm in A, B, G, and N; 50 μm in C–F, H–M, O, and P).